E-GEOD-45202 - Amplitude modulation of androgen signaling by c-MYC [RNA-Seq]

Status
Released on 16 March 2013, last updated on 24 March 2013
Organism
Homo sapiens
Samples (3)
Protocols (4)
Description
Androgen-stimulated growth of the molecular apocrine breast cancer is mediated by an androgen receptor (AR)-regulated transcriptional program. Through profiling the genomic licalizations of AR and its co-regulators FOXA1 and TCF7L2 in MDA-MB-453 breast cancer cells, we revealed the molecular details of the AR-centered regulatory network. We further identified that c-MYC is a key downstream target co-regulated by AR, FOXA1 and TCF7L2, and reinforces the transctiopnal activation of androgen-responsive genes in this subtype of breast cancers. MDA-MB-453 breast cancer cells were transfected with control of MYC siRNA for 48 h, followed by treatment with 10nM DHT or vehicle for 6 h. The cells were subjected to mRNA purification and library praparation for RNA-seq on Illumina HiSeq2000 platform.
Experiment type
RNA-seq of coding RNA 
Contacts
Yiwen Chen <ywchen@jimmy.harvard.edu>, Min Ni, Myles Brown, Teng Fei
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-45202.idf.txt
Sample and data relationshipE-GEOD-45202.sdrf.txt
Links