E-GEOD-45201 - Amplitude modulation of androgen signaling by c-MYC [ChIP-Seq]

Status
Released on 16 March 2013, last updated on 24 March 2013
Organism
Homo sapiens
Samples (6)
Protocols (4)
Description
Androgen-stimulated growth of the molecular apocrine breast cancer is mediated by an androgen receptor (AR)-regulated transcriptional program. Through profiling the genomic licalizations of AR and its co-regulators FOXA1 and TCF7L2 in MDA-MB-453 breast cancer cells, we revealed the molecular details of the AR-centered regulatory network. We further identified that c-MYC is a key downstream target co-regulated by AR, FOXA1 and TCF7L2, and reinforces the transctiopnal activation of androgen-responsive genes in this subtype of breast cancers. AR and FOXA1 ChIP-seq were performed in MDA-MB-453 breast cancer cells with treatment of 5a-dihydrotestosterone (DHT) for 16 h. TCF7L2 ChIP-seq was performed in MDA-MB-453 cells treated with vehicle or DHT for 16 h, respectively. MYC ChIP-seq was performed in MDA-MB-453 cells following 6 h DHT stimulation.
Experiment type
ChIP-seq 
Contacts
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-45201.idf.txt
Sample and data relationshipE-GEOD-45201.sdrf.txt
Processed data (1)E-GEOD-45201.processed.1.zip
Links