E-GEOD-45171 - Gene expression profile of endothelial cells maturated into brain-like endothelial cells

Status
Released on 18 October 2013, last updated on 13 May 2014
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (7)
Description
To understand the molecular mechanisms during the maturation of cord blood-derived endothelial cells into blood brain barrier capillary endothelial cells (BCECs), we have employed whole genome microarray expression profiling to identify genes responsible for the maturation process. Hematopoietic stem cells were isolated from cord-blood samples and differentiated into endothelial cells. The endothelial cells were further maturated into BCECs by co-culturing with blood-brain barrier (BBB) specific cells (pericytes) for 3 days and 6 days. The gene expression in human hematopoietic stem cell-derived endothelial cells was measured at 3 and 6 days after co-culture with pericytes. Three independent experiments were performed at each time (3 or 6 days). The RNA obtained from different experiments were pooled together for each group before microarray studies.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-45171.idf.txt
Sample and data relationshipE-GEOD-45171.sdrf.txt
Raw data (1)E-GEOD-45171.raw.1.zip
Processed data (1)E-GEOD-45171.processed.1.zip
Array designA-AGIL-28.adf.txt
Links