E-GEOD-45136 - Identification of the chemokine CCL28 as a growth and survival factor for human hematopoietic stem- and progenitor cells

Status
Released on 3 June 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (9)
Array (1)
Protocols (7)
Description
To discover novel growth factors for hematopoietic stem- and progenitor cells (HSPCs), we have assessed cytokine responses of cord blood (CB)-derived CD34+ cells in a high-content growth factor screen. We identify the immunoregulatory chemokine (C-C motif) ligand 28 (CCL28) as a novel growth factor that directly stimulates proliferation of primitive hematopoietic cells from different ontogenetic origins. Microarrays are used to compare the expression profiles of HSPCs expanded in SCF, TPO and CCL28 respectively. HSPCs were cultured in Serum-Free Expansion Medium with SCF, SCF + TPO, and SCF + CCL28 respectively, and hybridised microarrays in triplicate.
Experiment type
transcription profiling by array 
Contacts
Shamit Soneji <s.soneji@ucl.ac.uk>, C Karlsson, L Larsson, S Soneji
Citation
Identification of the chemokine CCL28 as a growth and survival factor for human hematopoietic stem and progenitor cells. Karlsson C, Baudet A, Miharada N, Soneji S, Gupta R, Magnusson M, Enver T, Karlsson G, Larsson J. , Europe PMC 23509159
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-45136.idf.txt
Sample and data relationshipE-GEOD-45136.sdrf.txt
Raw data (1)E-GEOD-45136.raw.1.zip
Processed data (1)E-GEOD-45136.processed.1.zip
Array designA-AFFY-44.adf.txt
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