E-GEOD-45105 - H3K9me2-Ab2 (new lot 3).D.mel 3rd Instar Larvae Nuclei G9a mutant.Affy 2

Status
Released on 13 March 2013, last updated on 3 May 2014
Organism
Drosophila melanogaster
Samples (4)
Array (1)
Protocols (5)
Description
modENCODE_submission_5618 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: G9a mutant (official name : G9a mutant genotype : G9a[RG5]/G9a[RG5] outcross : 3 transgene : n/a tags : homologous recombination tag : none description : Mutation generated by end-out homologous recombination at the G9a locus. The entire open reading frame of G9a has been removed. Developmental Stage: 3rd Instar Larvae; Genotype: G9a[RG5]/G9a[RG5]; Transgene: n/a; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain G9a mutant (official name : G9a mutant genotype : G9a[RG5]/G9a[RG5] outcross : 3 transgene : n/a tags : homologous recombination tag : none description : Mutation generated by end-out homologous recombination at the G9a locus. The entire open reading frame of G9a has been removed. Antibody H3K9me2-Ab2 (new lot 3) (target is H3K9me2); Developmental Stage 3rd Instar Larvae
Experiment type
ChIP-chip by tiling array 
Contacts
DCC modENCODE <help@modencode.org>, Aki Minoda, Andrey Gortchakov, Art Alekseyenko, Cameron Kennedy, Gary Karpen, Greg Shanower, Michael Tolstorukov, Mitzi Kuroda, Nicole Riddle, Peter Kharchenko, Peter Park, Sarah Elgin, Vincenzo Pirrotta, Yuri Schwartz
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-45105.idf.txt
Sample and data relationshipE-GEOD-45105.sdrf.txt
Raw data (1)E-GEOD-45105.raw.1.zip
Array designA-AFFY-146.adf.txt
Links