E-GEOD-4508 - Transcription profiling of mouse oocytes reveals abundant transcripts from retrotransposons are unstable

Status
Submitted on 21 March 2006, released on 13 June 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (2)
Description
One physiological function proposed for RNA interference (RNAi) is to constrain expression of repetitive elements and thereby reduce the incidence of retrotransposition. Consistent with this model is that inhibiting the RNAi pathway results in an increase in expression of repetitive elements in preimplantation mouse embryos. Mouse oocytes are essentially transcriptionally quiescent providing a unique opportunity to assess the stability of repetitive element-derived transcripts in these cells. We compared the transcriptome of freshly isolated fully-grown germinal vesicle (GV)-intact oocytes to that of oocytes in which meiotic maturation in vitro was inhibited for 48 h by milrinone. Consistent with the aforementioned function for RNAi is that the abundance of only a relatively small number of transcripts decreased in the cultured oocytes, when compared to changes that occur during maturation or following fertilization, and of those, several belonged to mobile elements. Experiment Overall Design: untreated GV oocytes (four replicates), GV oocytes cultured in milrinome for 48h (four replicates)
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Abundant transcripts from retrotransposons are unstable in fully grown mouse oocytes. Mareike Puschendorf, Paula Stein, Edward J Oakeley, Richard M Schultz, Antoine H F M Peters, Petr Svoboda. Biochem Biophys Res Commun 347(1):36-43 (2006)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-4508.idf.txt
Sample and data relationshipE-GEOD-4508.sdrf.txt
Processed data (1)E-GEOD-4508.processed.1.zip
Array designA-AFFY-45.adf.txt
Links