E-GEOD-45023 - Genome-wide maps of chromatin state for H3.3 tagged cells

Status
Released on 18 April 2013, last updated on 25 April 2013
Organism
Homo sapiens
Samples (3)
Protocols (5)
Description
The HIRA chaperone complex, comprised of HIRA, UBN1 and CABIN1, collaborates with histone-binding protein ASF1a to incorporate histone variant H3.3 into chromatin in a DNA replication-independent manner. To better understand its function and mechanism, we integrated HIRA, UBN1, ASF1a and histone H3.3 ChIP-seq and gene expression analyses. Most HIRA-binding sites co-localize with UBN1, ASF1a and H3.3 at active promoters and active and weak/poised enhancers. At promoters, binding of HIRA/UBN1/ASF1a correlates with the level of gene expression. HIRA is required for deposition of histone H3.3 at its binding sites. There are marked differences in nucleosome and co-regulator composition at different classes of HIRA-bound regulatory site. Underscoring this, we report novel physical interactions between the HIRA complex and transcription factors, a chromatin insulator and an ATP-dependent chromatin-remodelling complex. Our results map the distribution of the HIRA chaperone across the chromatin landscape and point to different interacting partners at functionally distinct regulatory sites. Examination of H3.3 histone modification in HeLA cells with accompanying FAIRE data
Experiment types
other, ChIP-seq 
Contacts
Tony McBryan <tony@mcbryan.co.uk>, Nikolay A Pchelintsev, Peter D Adams
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
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