E-GEOD-44765 - Global profiling of human hair follicle scalp dermal papilla cells using Affymetrix microarrays

Status
Released on 20 October 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (18)
Array (1)
Protocols (7)
Description
Dermal papilla cells isolated from the human hair follicle are capable of inducing hair growth in recipient epithelia. However, demonstrating disparity from rodent dermal papilla, human cells lose this inductive competance immediately upon growth in culture under normal growth conditions. We grew dermal papilla cells in hanging drop cultures that are morphologically akin to intact dermal papilla, and found that by enhancing the environment for aggregation, we could restore the inductive capacity of human dermal papilla cells in culture. The underlying genes that regulate the inductive potential of dermal papilla cells is not well understood, and we sought to use global profiling to identify key genes and pathways related to inductive competance within dermal papilla cells. We used Affymetrix microarrays to profile human dermal papilla cells in both hair inducing, and non-hair inducing states. Affymetrix microarrays were used to to perform profiling of human dermal papilla cells, both as intact tissues (freshly isolated from scalp), and at several stages in subsequent two dimensional culture; cell explant outgrowths (p0), cells at passage 1 (p1), passage 3 (p3) and passage 5 (p5). RNA was isolated from cultured cells 72 hours after feeding. Cells at passage 3 were also grown in hanging drops to form dermal spheroids, that were used for RNA collection 48 hours after establishment. All experiments were performed using tissue from three biological replicates (#D5, D6, D7),
Experiment type
transcription profiling by array 
Contacts
Angela M Christiano, Claire A Higgins
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-44765.idf.txt
Sample and data relationshipE-GEOD-44765.sdrf.txt
Raw data (1)E-GEOD-44765.raw.1.zip
Processed data (1)E-GEOD-44765.processed.1.zip
Array designA-AFFY-44.adf.txt
Links