E-GEOD-44764 - The histone demethylase Jmjd3 sequentially associates with the transcription factors Tbx3 and Eomes to drive endoderm differentiation

Status
Released on 18 April 2013, last updated on 29 April 2013
Organism
Mus musculus
Samples (4)
Protocols (4)
Description
Here we show that T-box proteins team up with chromatin modifying enzymes to drive the expression of the key lineage regulator, Eomes during endodermal differentiation of embryonic stem (ES) cells. The Eomes locus is maintained in a transcriptionally poised configuration in ES cells. During early differentiation steps, the ES cell factor Tbx3 associates with the histone demethylase Jmjd3 at the enhancer element of the Eomes locus to allow enhancer-promoter interactions. This spatial reorganization of the chromatin primes the cells to respond to Activin signaling, which promotes the binding of Jmjd3 and Eomes to its own bivalent promoter region to further stimulate Eomes expression in a positive feedback loop. Examination of the binding of pluripotency factors to mouse embryonic stem cells and embryoid bodies
Experiment type
ChIP-seq 
Contacts
April Kartikasari <akartikasari@mednet.ucla.edu>, A Bhushan, A E Kartikasari, A Grapin-Botton, A Sinha, D N Chan, J X Zhou, M Magnuson, M S Kanji, W E Lowry
Citation
The histone demethylase Jmjd3 sequentially associates with the transcription factors Tbx3 and Eomes to drive endoderm differentiation. Kartikasari AE, Zhou JX, Kanji MS, Chan DN, Sinha A, Grapin-Botton A, Magnuson MA, Lowry WE, Bhushan A. , Europe PMC 23584530
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
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