E-GEOD-4475 - Transcription profiling of human Burkitts lymphomas

Status
Submitted on 15 March 2006, released on 13 June 2008, last updated on 27 March 2012
Organism
Homo sapiens
Samples (221)
Array (1)
Protocols (2)
Description
The distinction between the Burkitt lymphoma and diffuse large B-cell lymphoma is imprecise using current diagnostic criteria. We applied transcriptional and genomic profiling to molecularly define Burkitt lymphoma. Gene expression profiling employing Affymetrix GeneChips (U133A) was performed in 220 mature aggressive B-cell lymphomas, including a core group of eight Burkitt lymphomas, which fulfilled all diagnostic criteria of the WHO classification. A molecular signature of Burkitt lymphoma was generated. Chromosomal abnormalities were detected by interphase fluorescence in-situ hybridization and array comparative genomic hybridization. The molecular Burkitt lymphoma signature identified 44 cases. Fifteen of these cases lacked a morphology typical for Burkitt/Burkitt-like lymphoma. The vast majority (88%) of the 176 lymphomas without the molecular Burkitt lymphoma signature represented diffuse large B-cell lymphomas. In 20% of these cases a MYC break was detectable which was associated with complex chromosomal changes. Our molecular definition of Burkitt lymphoma sharpens and extends the spectrum of Burkitt lymphoma. In mature aggressive B-cell lymphomas without a Burkitt lymphoma signature, a chromosomal break in the MYC locus proved to be associated with adverse clinical outcome. Experiment Overall Design: 220 diffuse large B-cell lymphoma and Burkitt lymphoma samples hybridized to 221 HGU133A Affymetrix GeneChips
Experiment types
transcription profiling by array, unknown experiment type
Contact
Michael Hummel
Citation
A biologic definition of Burkitt's lymphoma from transcriptional and genomic profiling. Michael Hummel, Stefan Bentink, Hilmar Berger, Wolfram Klapper, Swen Wessendorf, Thomas F E Barth, Heinz-Wolfram Bernd, Sergio B Cogliatti, Judith Dierlamm, Alfred C Feller, Martin-Leo Hansmann, Eugenia Haralambieva, Lana Harder, Dirk Hasenclever, Michael Kühn, Dido Lenze, Peter Lichter, Jose Ignacio Martin-Subero, Peter Möller, Hans-Konrad Müller-Hermelink, German Ott, Reza M Parwaresch, Christiane Pott, Andreas Rosenwald, Maciej Rosolowski, Carsten Schwaenen, Benjamin Stürzenhofecker, Monika Szczepanowski, Heiko Trautmann, Hans-Heinrich Wacker, Rainer Spang, Markus Loeffler, Lorenz Trümper, Harald Stein, Reiner Siebert. N Engl J Med 354(23):2419-30 (2006)
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