7 protocols
AccessionType
normalization data transformation protocol
Median normalized values derived from Codelink Expression Analysis Version 5.0 ID_REF = VALUE = Normalized signal intensities
array scanning protocol
Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
hybridization protocol
Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
labelling protocol
Codelink Gene Expression System: Manual Labelled cRNA Target Preparation according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/iexpress2006_web.pdf
sample treatment protocol
Three stallions were treated with 0.1 mg/kg dexamethasone i.v. while three control stallions received an equivalent volume of saline. Testes were collected 12 h post-injection and assessed for differential gene expression
nucleic acid extraction protocol
Tripure reagent was used to prepare total RNA from minced parenchymal samples of testes from each stallion
growth protocol
Adult stallions fed free choice hay and moderate amount of horse feed