7 protocols
AccessionNameType
P-GSE44736-1
normalization data transformation protocol
Median normalized values derived from Codelink Expression Analysis Version 5.0 ID_REF = VALUE = Normalized signal intensities
P-GSE44736-7
array scanning protocol
Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
P-GSE44736-6
hybridization protocol
Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
P-GSE44736-5
labelling protocol
Codelink Gene Expression System: Manual Labelled cRNA Target Preparation according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/iexpress2006_web.pdf
P-GSE44736-2
sample treatment protocol
Three stallions were treated with 0.1 mg/kg dexamethasone i.v. while three control stallions received an equivalent volume of saline. Testes were collected 12 h post-injection and assessed for differential gene expression
P-GSE44736-4
nucleic acid extraction protocol
Tripure reagent was used to prepare total RNA from minced parenchymal samples of testes from each stallion
P-GSE44736-3
growth protocol
Adult stallions fed free choice hay and moderate amount of horse feed