E-GEOD-44693 - MiR-155 promotes blood-brain barrier dysfunction in neuroinflammation (part 2)
Released on 29 April 2014, last updated on 2 June 2014
Over-expression of miR-155 induces changes in the pattern of gene expression of hCMEC/D3 cells. hypothesis tested in the present study was that miR-155 constitute an important regulatory control of the brain endothelial response to inflammatory cytokines. To identify miR-155 target genes in brain endothelim that might be implicated in BBB dysfunction relevant to human disease, we then analysed changes in mRNA expression of hCMEC/D3 cells that overexpress miR-155 and results were contrasted to cells transfected with scrambled miR. To ectopically express miR-155 in hCMEC/D3 cells, 30 nM of pre-miR-155 and the siPORT Amine transfection agent (Applied Biosystems, Warrington, UK) were combined following the manufacturer’s instructions.
transcription profiling by array
Miguel Alejandro Lopez-Ramirez <firstname.lastname@example.org>, Arie Reijerkerk, Basil Sharrack, David Baker, David K Male, Dongsheng Wu, Gareth Pryce, Gregory J Michael, Helga E de Vries, Ignacio A Romero, Josh King-Robson, Julie E Simpson, Mark C Hirst, Miguel A Lopez-Ramirez, Oliver Kay