E-GEOD-44686 - Genome-wide analysis of dFOXO binding sites in Drosophila

Status
Released on 20 September 2013, last updated on 3 October 2013
Organism
Drosophila melanogaster
Samples (8)
Protocols (2)
Description
To understand how reduced insulin/IGF-1 signaling extends Drosophila lifespan through its downstream transcription factor dFOXO. We conducted ChIP analysis with a dFOXO antibody followed by Illumina high-throughput sequencing from chico heterozygous mutants, which are long-lived and normal sized, and from adult flies with ablated insulin producing cells (IPCs), which are also long-lived. dFOXO bound at promoters of 273 genes common among these genotypes, thus potentially enriching for shared factors in control of aging. Two replicates were sequenced from chico heterozygous mutants and IPC ablated flies.
Experiment type
ChIP-seq 
Contacts
Hua Bai, Marc Tatar
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-44686.idf.txt
Sample and data relationshipE-GEOD-44686.sdrf.txt
Links