E-GEOD-4462 - Transcription profiling of mouse renal epithelial cells from inv mutant animals

Status
Submitted on 14 March 2006, released on 13 June 2008, last updated on 27 March 2012
Organism
Mus musculus
Samples (2)
Array (1)
Description
Nephronophthisis (NPHP) is a group of progressive renal disorders characterized by a variable number of cysts associated with cortical tubular atrophy. Recently, mutations in INV have been identified to be responsible for NPHP2. The inv/inv,inv-deltaC::GFP mouse that was created by introduction of inv gene lacking the C-terminus (inv-deltaC) into inv/inv mice develops multiple renal cysts without situs abnormality. Renal cyst progression was dependent on expression level of inv-deltaC transgene, suggesting that inv protein functions in a dose-dependent manner. Cell proliferation and apoptosis were increased in the inv-deltaC cystic kidneys. We searched up- and down-regulated genes during cyst development and progression using cDNA microarray. See appended table for comparison analysis of inv/inv,inv-deltaC::GFP vs +/+,inv-deltaC::GFP (GSM100334 vs GSM100335). Experiment Overall Design: Comparison of gene expression in each two kidneys in 4-week-old inv-deltaC and control mice was carried out by the KURABO Industries, Biomedical Department
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Sustained cell proliferation of renal epithelial cells in mice with inv mutation. Noriyuki Sugiyama, Takahiko Yokoyama. Genes Cells 11(10):1213-24 (2006)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-4462.idf.txt
Sample and data relationshipE-GEOD-4462.sdrf.txt
Processed data (1)E-GEOD-4462.processed.1.zip
Array designA-AFFY-45.adf.txt
Links