E-GEOD-44458 - Bone marrow-derived multipotent mesenchymal stromal cells promote tongue cancer invasion by inducing collagen production

Status
Released on 1 December 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (5)
Description
Tumor microenvironment (TME) is an active player in malignant growth and spread. Changes in the composition and structure of TME and extracellular matrix can result in either suppression or facilitation of malignant tumor growth. Carcinoma‐associated fibroblasts, bone marrow-derived multipotent mesenchymal stromal cells (BMMSCs), tumor associated macrophages and other inflammatory cells all affect the composition of TME, proliferation and survival of cancer cells, angiogenesis, invasion and metastasis. The objective of this work was to investigate the effect of the interaction between bone marrow-derived BMMSCs and human oral tongue squamous cell carcinoma (OTSCC) cells in the processes of invasion and gene expression. Co-cultures of OTSCC cancer cells and BMMSCs in 3D organotypic invasion assay were used in addition to cell culture, immunological, microarray, and RNA interference techniques. Total number of 4 samples were analyzed. 2 replicates of cultured human oral tongue squamous cell carcinoma (OTSCC) cells, and 2 replicates of OTSCC cells co-cultured with bone marrow-derived multipotent mesenchymal stromal cells
Experiment type
transcription profiling by array 
Contacts
Jussi Vuoristo <geo@ncbi.nlm.nih.gov>, Carolina Bitu, Daniela E Costea, Hannu Vähänikkilä, Joonas Kauppila, Jussi T Vuoristo, Kalle Merkku, Meeri Sutinen, Petri Lehenkari, Pia Nyberg, Siri Lehtonen, Sirpa Salo, Tuula Salo
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-44458.idf.txt
Sample and data relationshipE-GEOD-44458.sdrf.txt
Raw data (1)E-GEOD-44458.raw.1.zip
Processed data (1)E-GEOD-44458.processed.1.zip
Array designA-AFFY-44.adf.txt
Links