E-GEOD-44399 - Analysis of Drosophila salivary glands and Kc cells with depleted levels of linker histone H1 (Illumina smRNA-Seq]
Released on 22 April 2013, last updated on 29 April 2013
Salivary glands or larval ovaries were isolated from transgenic flies expressing RNAi targeting Nautilus (control) or linker histone H1 using a Tub-Gal4 driver. ~200 larvae were used to isolate salivary glands or ovaries, independently. Total RNA was isolated using Trizol reagent following manufacturer's guidelines. Then 5 µg of total RNA was separated on a polyacrylamide gel, and 18-29 nt small RNAs were isolated for cloning.
RNA-seq of non coding RNA
Sandeep Nagaraj Wontakal <email@example.com>, Arthur Skoultchi, Xingwu Lu
Drosophila H1 regulates the genetic activity of heterochromatin by recruitment of Su(var)3-9. Lu X, Wontakal SN, Kavi H, Kim BJ, Guzzardo PM, Emelyanov AV, Xu N, Hannon GJ, Zavadil J, Fyodorov DV, Skoultchi AI. , Europe PMC 23559249