E-GEOD-44399 - Analysis of Drosophila salivary glands and Kc cells with depleted levels of linker histone H1 (Illumina smRNA-Seq]

Status
Released on 22 April 2013, last updated on 29 April 2013
Organism
Drosophila melanogaster
Samples (4)
Protocols (4)
Description
Salivary glands or larval ovaries were isolated from transgenic flies expressing RNAi targeting Nautilus (control) or linker histone H1 using a Tub-Gal4 driver. ~200 larvae were used to isolate salivary glands or ovaries, independently. Total RNA was isolated using Trizol reagent following manufacturer's guidelines. Then 5 µg of total RNA was separated on a polyacrylamide gel, and 18-29 nt small RNAs were isolated for cloning.
Experiment type
RNA-seq of non coding RNA 
Contacts
Citation
Drosophila H1 regulates the genetic activity of heterochromatin by recruitment of Su(var)3-9. Lu X, Wontakal SN, Kavi H, Kim BJ, Guzzardo PM, Emelyanov AV, Xu N, Hannon GJ, Zavadil J, Fyodorov DV, Skoultchi AI. , Europe PMC 23559249
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-44399.idf.txt
Sample and data relationshipE-GEOD-44399.sdrf.txt
Processed data (1)E-GEOD-44399.processed.1.zip
Links