E-GEOD-44368 - The human placental sexome differs between trophoblast epithelium and villous vessel endothelium

Status
Released on 1 October 2013, last updated on 7 October 2013
Organism
Homo sapiens
Samples (32)
Array (1)
Protocols (7)
Description
As susceptibility to many adult disorders originates in utero, we here hypothesized that fetal sex influences gene expression in placental cells and produces functional differences in human placentas. We found that fetal sex differentially affects gene expression in a cell-phenotype dependent manner among all four placental cell-phenotypes studied: cytotrophoblasts, syncytiotrophoblasts, arterial endothelial cells and venous endothelial cells. The markedly enriched pathways in males were identified to be signaling pathways for graft-versus-host disease as well as the immune and inflammatory systems, both supporting the hypothesis that there is reduced maternal-fetal compatibility for male fetuses. Our study is the first microarray study investigating sexual dimorphism in purified and characterized somatic cells from a single human tissue, the placenta, that underlines the importance of considering fetal sex as an independent variable in any work using human placenta. Arterial and venous endothelial cells were isolated from eight different placentas, four of each sex. A total of ten placentas were used for isolation of cytotrophoblasts and six for syncytiotrophoblasts, with equal numbers from each sex.
Experiment type
transcription profiling by array 
Contacts
Gernot Desoye, Hubert Hackl, Karin Wagner, Mark Longtine, Michael D Nelson, Silvija Cvitic, Ursula Hiden
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-44368.idf.txt
Sample and data relationshipE-GEOD-44368.sdrf.txt
Raw data (1)E-GEOD-44368.raw.1.zip
Processed data (1)E-GEOD-44368.processed.1.zip
Array designA-AFFY-141.adf.txt
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