E-GEOD-44363 - Transcriptomic modulation by adenosine 2A receptors in normal and endotoxemic myocardium

Status
Released on 1 May 2013, last updated on 2 June 2014
Organism
Mus musculus
Samples (16)
Array (1)
Protocols (7)
Description
Analysis of venticular myocardium from adenosine 2A receptors (A2AR) knockouts following LPS stimulation. Results provide insight into the molecular components of A2AR mediated protection, but also reveal pathogenetic components of endotoxemic myocarditis as a result of LPS exposure. These findings demonstrate that intrinsic A2AR activity exerts limited transcriptional effects in unstressed heart, modifying G-coupled cAMP/PKA signal paths. LPS-dependent injury and dysfunction is associated with profound up-regulation of inflammatory/immune processes, fibrotic and cell death paths, and NF-kB, Erk/MAPK and JAK/Stat signaling, with shifts in multiple determinants of cardiac contraction and survival. Intrinsic A2AR activity modulates key aspects of these inflammatory responses, involving MAPK, JAK/Stat and NF-kB signaling Total RNA obtained from adenosine 2A receptor knockout or wild-type murine ventricular myocardium that were treated for 24 hours with either saline or lipopolysaccharide (n=4/group).
Experiment type
transcription profiling by array 
Contacts
Kevin John Ashton <keashton@bond.edu.au>, Bunyen Teng, Catherine Ledent, John P Headrick, Kevin J Ashton, Lea Delbridge, Melissa E Reichelt, Polly A Hofmann, R R Morrison, S J Mustafa
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-44363.idf.txt
Sample and data relationshipE-GEOD-44363.sdrf.txt
Raw data (1)E-GEOD-44363.raw.1.zip
Processed data (1)E-GEOD-44363.processed.1.zip
Array designA-AFFY-45.adf.txt
Links