E-GEOD-4429 - Transcription profiling of Arabidopsis thaliana response to the bacterial pathogen Pseudomonas syringae ES4326
Submitted on 9 March 2006, released on 13 June 2008, last updated on 27 March 2012
In order to protect themselves from pathogens, plants activate a battery of defense pathways, many of which involve changes in gene expression. We are interested in identifying plant genes that are differentially expressed in response to pathogen exposure, with the ultimate goal of studying the roles of these genes in plant defense. Experiment Overall Design: Whole plants are grown in soil, and individual leaves from 3-4 week old plants are injected with a bacterial suspension, or a mock (5mM MgSO4) control. Approximately five leaves are harvested 24 hours after inoculation and used for RNA preparation, labeling and Affymetrix hybridization.
transcription profiling by array, unknown experiment type
Raka Mustaphi Mitra
A high-performance, small-scale microarray for expression profiling of many samples in Arabidopsis-pathogen studies. Masanao Sato, Raka M Mitra, John Coller, Dong Wang, Natalie W Spivey, Julia Dewdney, Carine Denoux, Jane Glazebrook, Fumiaki Katagiri. Plant J 49(3):565-77 (2007)