E-GEOD-44188 - DamID of Dam::LMN-1 and Dam::EMR-1 in Caenorhabditis elegans adult worms

Status
Released on 2 December 2013, last updated on 9 December 2013
Organism
Caenorhabditis elegans
Samples (24)
Array (1)
Protocols (5)
Description
Laminopathies are caused by mutations in components of the nuclear envelope (NE). While most NE components are widely expressed, laminopathies affect only a subset of tissues. However, the understanding of the molecular mechanisms that explain this phenomenon is still elusive. Here we have performed a genome wide DamID analysis in adult C. elegans nematodes comparing the DNA association profile of two components of the NE, Lamin/LMN-1 and Emerin/EMR-1. Although both proteins were associated to silent DNA, EMR-1 showed a predominant role in the anchoring of muscle and neuronal promoters to the nuclear periphery. Deletion of either EMR-1 or LEM-2, another integral NE protein, caused local changes in nuclear architecture with both increased and decreased LMN-1 association. Comparison of Dam::LMN-1 and Dam::EMR-1 DNA assotiation in wild type strains and Dam::LMN-1 DNA association in wild type, lem-2(tm1582) and emr-1(gk119) mutant backgrounds.
Experiment type
ChIP-chip by tiling array 
Contacts
Cristina González-Aguilera, Peter Askjaer
MIAME
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