E-GEOD-44166 - Expression Data from Normal or ErbB2 Tumor Fibroblasts With or Without Ets2

Status
Released on 1 December 2013, last updated on 2 June 2014
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (7)
Description
The mechanisms involved in epithelium-stroma interactions remain poorly understood, despite the importance of the microenvironment during tumorigenesis. Here, we studied the role of Ets2 transcrpiton factor in tumor associated fibroblasts in the MMTV-ErbB2 mammary tumor model. Inactivation of Ets2 specifically in fibroblasts using Fsp-cre significantly reduced tumor growth, in contrast to Ets2 inactivation in epithelium in which no differences in tumor growth were observed. Microarray analysis on isolated fibroblasts demonstrated the important role of Ets2 in remodeling of the extracellular matrix and angiogenesis in these cells. Tumors lacking Ets2 in fibroblats had diminished blood vessels. Our tumor specific gene signature was also represented in the stroma of human breast cancer patients. Collectively, our results suggest Ets2 uniquely contributes to angiogenesis from fribroblasts in the tumor microenvironment. Primary mammary fibroblasts were isolated from mice with no oncogene with or without Ets2 and with the ErbB2 oncogene with or without Ets2, RNA was extracted and samples were submitted for Affymetrix gene expression arrays
Experiment type
transcription profiling by array 
Contacts
Julie Wallace <Julie.Wallace@osumc.edu>, Lianbo Yu, Michael C Ostrowski, Subhasree Balakrishnan
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-44166.idf.txt
Sample and data relationshipE-GEOD-44166.sdrf.txt
Raw data (1)E-GEOD-44166.raw.1.zip
Processed data (1)E-GEOD-44166.processed.1.zip
Array designA-AFFY-45.adf.txt
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