E-GEOD-44118 - Expression Data from Endothelial Cells as a Consequence of Ets2 Signaling in PyMT Tumor Associated Fibroblasts

Status
Released on 1 December 2013, last updated on 2 June 2014
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
Tumor associated fibroblasts are known to play an important role in angiogenesis, however the specific signaling pathways playing an important role in this cross talk remain ill defined. Here, we studied how Ets2 transcrpiton factor signaling in tumor associated fibroblasts effected gene expression in surrounding endothelial cells in the MMTV-PyMT mammary tumor model. Inactivation of Ets2 specifically in fibroblasts using Fsp-cre significantly reduced tumor associated angiogenesis, and was indicated to effect ECM remodeling, cell adhesion and cell chemotaxis processes in neighboring endothelial cells. We have identified Ets2 in fibroblasts as a key signaling molecule to induce tumor associated angiogenesis. Additionally, this function does not rely on the presence of tumor cells, indicating a memory in fibroblasts to induce angiogenesis. Primary mammary endothelial cells were isolated from mice with or without fibroblast Ets2 in the presence of the PyMT oncogene through sorting with a CD31 antibody. RNA was extracted and samples were submitted for Affymetrix gene expression arrays
Experiment type
transcription profiling by array 
Contacts
Julie Wallace, Lianbo Yu, Michael C Ostrowski
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-44118.idf.txt
Sample and data relationshipE-GEOD-44118.sdrf.txt
Raw data (1)E-GEOD-44118.raw.1.zip
Processed data (1)E-GEOD-44118.processed.1.zip
Array designA-AFFY-45.adf.txt
Links