E-GEOD-44116 - Cytosolic Fe-S cluster assembly-deficient mutant, nar1 and nbp35 mutant seedling

Status
Released on 1 May 2013, last updated on 13 May 2014
Organism
Arabidopsis thaliana
Samples (6)
Array (1)
Protocols (7)
Description
Expression profiling of two-weeks-old wild type, nar1-4/- and nbp35-3/- mutant seedlings. The cytosolic Fe -S cluster assembly pathway is involved in cytosolic and nucleus Fe-S protein maturation. The nar1 mutant was identified by the screeing for abnormal transcription feature in endosperm.We performed genome-wide transcriptional profiling of weak allele nar1-4/- using microarrays to estimate whether the cytosolic Fe -S cluster assembly could affect on the transcriptional profile even in vegetative tissues. Two biological replicate were performed using two weeks seedling of wild type, nar1-4/- and nbp35-3/-. RNA was extracted using the RNeasy mini kit(QIAGEN).
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-44116.idf.txt
Sample and data relationshipE-GEOD-44116.sdrf.txt
Raw data (1)E-GEOD-44116.raw.1.zip
Processed data (1)E-GEOD-44116.processed.1.zip
Array designA-AFFY-2.adf.txt
Links