E-GEOD-44048 - Human glioma cell line T98G: BAP-PCBP2 RIP vs. BAP-GFP RIP (Control)

Status
Released on 24 July 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (8)
Description
RIP-Chip analysis of PCBP2 and identification of preferentially associated mRNAs. T98G cells were transfected transiently with BAP-tagged constructs. BAP-tagged proteins were biotinylated in vivo by the co-transfected hBirA enzyme. RNPs were recovered via precipitation with Steptavidin-sepharose beads. Finally, RNAs were purified and analyzed on microarrays. BAP-GFP as control was used in three independent sets of experiments. Two-condition experiment, BAP-PCBP2 vs. BAP-GFP cells. Biological replicates: 3 BAP-PCBP2 replicates, 3 BAP-GFP (Control) replicates
Experiment type
transcription profiling by array 
Contacts
Citation
RNA-binding protein PCBP2 modulates glioma growth by regulating FHL3. Han W, Xin Z, Zhao Z, Bao W, Lin X, Yin B, Zhao J, Yuan J, Qiang B, Peng X. , Europe PMC 23585479
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-44048.idf.txt
Sample and data relationshipE-GEOD-44048.sdrf.txt
Raw data (1)E-GEOD-44048.raw.1.zip
Processed data (1)E-GEOD-44048.processed.1.zip
Array designA-AGIL-28.adf.txt
Links