E-GEOD-44048 - Human glioma cell line T98G: BAP-PCBP2 RIP vs. BAP-GFP RIP (Control)

Status
Released on 24 July 2013, last updated on 19 August 2015
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (8)
Description
RIP-Chip analysis of PCBP2 and identification of preferentially associated mRNAs. T98G cells were transfected transiently with BAP-tagged constructs. BAP-tagged proteins were biotinylated in vivo by the co-transfected hBirA enzyme. RNPs were recovered via precipitation with Steptavidin-sepharose beads. Finally, RNAs were purified and analyzed on microarrays. BAP-GFP as control was used in three independent sets of experiments. Two-condition experiment, BAP-PCBP2 vs. BAP-GFP cells. Biological replicates: 3 BAP-PCBP2 replicates, 3 BAP-GFP (Control) replicates
Experiment type
transcription profiling by array 
Contacts
Citation
RNA-binding protein PCBP2 modulates glioma growth by regulating FHL3. Han W, Xin Z, Zhao Z, Bao W, Lin X, Yin B, Zhao J, Yuan J, Qiang B, Peng X.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-44048.idf.txt
Sample and data relationshipE-GEOD-44048.sdrf.txt
Raw data (1)E-GEOD-44048.raw.1.zip
Processed data (1)E-GEOD-44048.processed.1.zip
Array designA-AGIL-28.adf.txt
Links