4 protocols
AccessionNameType
P-GSE43966-4
normalization data transformation protocol
Sequence tags were processed and analyzed using BioScope software package (Applied Biosystems) MicroRNA sequence tags were normalized by thousand mappable tags Genome_build: hg19 Supplementary_files_format_and_content: Tab delimited file of microRNA sequence tags per thousand mappable tags
P-GSE43966-3
nucleic acid library construction protocol
RNA was purified and size fractionated using miRNEASY RNA isolation kit and Rneasy MinElute Clean up kit (Qiagen) Libraries were constructed using 1 microgram of small RNA and the Small RNA Expression Kit (Applied Biosystems)
P-GSE43966-1
sample treatment protocol
Cells were either mock infected or infected with Sendai virus (5 pfu/cell) for 10 hours
P-GSE43966-2
growth protocol
A549 cells were cultured in F12K media (Gibco) supplemented with 10% cosmic calf serum (Hyclone) and pennecilin and streptomycin