E-GEOD-43805 - TMEM88 knockdown during human embryonic stem cell cardiac differentiation

Status
Released on 21 October 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (6)
Description
TMEM88 is indispensable for heart development and acts in the pre-cardiac mesoderm to specify lineage commitment of the cardiovascular progenitor cell through inhibition of Wnt signaling. 2 different lentiviruses expression shRNA targeting different domains of the TMEM88 locus were transduced into undifferentiated hES cells. Cells were puromycin selected then differentiated along the cardiac lineage. Total RNA was taken at day 5 of differentiation when the cardiovascular progenitor cell arises. 1 sample for each shRNA and 2 samples for control shRNA were analyzed by array
Experiment type
transcription profiling by array 
Contacts
Nathan Palpant <npalpant@uw.edu>, Charles E Murry, Nathan J Palpant
Citation
Transmembrane protein 88: a Wnt regulatory protein that specifies cardiomyocyte development. Palpant NJ, Pabon L, Rabinowitz JS, Hadland BK, Stoick-Cooper CL, Paige SL, Bernstein ID, Moon RT, Murry CE. , Europe PMC 23924634
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-43805.idf.txt
Sample and data relationshipE-GEOD-43805.sdrf.txt
Processed data (1)E-GEOD-43805.processed.1.zip
Additional data (1)E-GEOD-43805.additional.1.zip
Array designA-GEOD-10558.adf.txt
Links