E-GEOD-43411 - NanoString miRNA profiling of THP1-differentiated macrophages treated with LPS derived from A. aggregatibacter, P. gingivalis and Pg LPS treated with cigrate smoke extract (Pg CSE)

Status
Released on 19 September 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (7)
Description
To examine the early (4h) miRNAs responses of differentiated macrophages to LPS challenge, we performed global miRNA profiling using the Nanostring nCounter technology, a multiplexed, color-coded probe assay. Of the 653 probes present on the assay, we detected expression of ~200 (30%) miRNAs across all samples. THP-1 differentiated cells were treated in duplicates with Aa, Pg and Pg (CSE) LPS for 4h and the cells were harvested after washing twice with PBS. Total RNA was isolated using miRNeasy kit (Qiagen). Samples were then processed for Nanostring profiling.
Experiment type
transcription profiling by array 
Contacts
Afsar Raza Naqvi <afsarrazaa@yahoo.co.in>, Afsar R Naqvi, Jezrom Self-Fordham, Salvador Nares
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-43411.idf.txt
Sample and data relationshipE-GEOD-43411.sdrf.txt
Raw data (1)E-GEOD-43411.raw.1.zip
Processed data (1)E-GEOD-43411.processed.1.zip
Array designA-GEOD-16486.adf.txt
Links