E-GEOD-43409 - RORγt+ Innate lymphoid cells transcriptomes after aNKp44 and cytokine stimulation

Status
Released on 30 October 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (11)
Array (1)
Protocols (6)
Description
RORγt+ innate lymphoid cells (ILC) are crucial players of innate immune responses and represent a major source of IL-22, which has an important role in mucosal homeostasis. The signals required by RORγt+ ILC to express IL-22 and other cytokines, including TNF, have only partially been elucidated. Here we show that RORγt+ ILC can directly sense the environment by the engagement of the activating receptor NKp44. NKp44 triggering in RORγt+ ILC selectively activates a coordinated pro-inflammatory program, including TNF, while cytokine stimulation induces preferentially IL-22 expression. However, combined engagement of NKp44 and cytokine receptors results in a strong synergistic effect. These data support the concept that NKp44+ RORγt+ ILC can be activated without cytokines and are able to switch between IL-22 or TNF production, depending on the triggering stimulus. Transcriptome analysis of CD56+CD127hi tonsil ILC, Ex vivo and after stimulation with aNKp44, IL-1/IL-7/IL-23 or aNKp44/IL-1/IL-7/IL-23 for 3.5h. RNA was extracted and pooled from 2 donors each, Amplified and labeled according to manufacturer´s instructions (GeneChipU133plus2® , Affymetrix). The analysis was performed in triplicates.
Experiment type
transcription profiling by array 
Contacts
Chiara Romagnani, Johannes Meisig, Monica Killig, Timor Glatzer
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-43409.idf.txt
Sample and data relationshipE-GEOD-43409.sdrf.txt
Raw data (1)E-GEOD-43409.raw.1.zip
Processed data (1)E-GEOD-43409.processed.1.zip
Array designA-AFFY-44.adf.txt
Links