E-GEOD-43275 - Genome-wide maps of Sox/Oct partnerships pluripotent and lineage-committed cells

Status
Released on 23 April 2013, last updated on 29 April 2013
Organism
Mus musculus
Samples (20)
Protocols (4)
Description
We show here by using genome-wide ChIP-sequencing that lineage segregation involves multiple Sox/Oct partnership. In undifferentiated ES cells Oct4 interacts with Sox2 and both TFs bind on the 'canonical' motif, whereas in cells commited to PrE lineage Oct4 switches from Sox2 to Sox17 interaction and this complex bind to a unique "compressed" motif. ChIP-sequencing has been done for Sox2, Sox17 and Oct4 in the pluripotent context or PrE context
Experiment type
ChIP-seq 
Contacts
Irene Aksoy, Larry Stanton
Citation
Oct4 switches partnering from Sox2 to Sox17 to reinterpret the enhancer code and specify endoderm. Aksoy I, Jauch R, Chen J, Dyla M, Divakar U, Bogu GK, Teo R, Leng Ng CK, Herath W, Lili S, Hutchins AP, Robson P, Kolatkar PR, Stanton LW. , Europe PMC 23474895
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-43275.idf.txt
Sample and data relationshipE-GEOD-43275.sdrf.txt
Processed data (2)E-GEOD-43275.processed.1.zip, E-GEOD-43275.processed.2.zip
Links