E-GEOD-43153 - Antiviral RNA interference in mammalian cells

Status
Released on 10 October 2013, last updated on 25 November 2013
Organism
Mus musculus
Samples (9)
Protocols (3)
Description
Plants and invertebrates protect themselves from viruses through RNA interference (RNAi), yet it remains unknown whether this defense mechanism exists in mammals. Antiviral RNAi involves the processing of viral long double-stranded (ds) RNA molecules into small interfering RNAs (siRNAs) by the ribonuclease (RNAse) III Dicer. These siRNAs are incorporated into effector complex(es) containing members of the Argonaute (Ago) protein family and guide silencing of complementary target viral RNAs. Here, we detect the accumulation of phased Dicer-dependent virus-derived siRNA (viRNAs) and demonstrate their loading into Ago2 after infection of mouse embryonic stem (ES) cells with Encephalomyocarditis virus (EMCV). We further show that the production of these viRNAs is drastically reduced, yet not completely abolished, if ES cells are first induced to differentiate before infection. Finally, we reveal that the mammalian virus Nodamura virus (NoV) encodes for a protein that counteracts such antiviral RNAi in ES cells supporting the existence of an effective RNAi-based immunity in mammals. Infection of wild-type or mutant mouse ES cells and analysis of small RNAs from total extracts or immunoprecipitated components of the RNAi pathway
Experiment types
other, RNA-seq of non coding RNA 
Contacts
Antonin Marchais, Constance Ciaudo, Florence Jay, Olivier Voinnet, Pierre V Maillard, Shou-Wei Ding, Yang Li
Citation
Antiviral RNA interference in mammalian cells. Maillard PV, Ciaudo C, Marchais A, Li Y, Jay F, Ding SW, Voinnet O. , Europe PMC 24115438
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-43153.idf.txt
Sample and data relationshipE-GEOD-43153.sdrf.txt
Processed data (1)E-GEOD-43153.processed.1.zip
Links