E-GEOD-4301 - Diverse and specific gene expression responses to stresses in cultured human cells
Submitted on 24 February 2006, released on 24 February 2006, last updated on 27 March 2012
TT3 and TN are cell cycle time courses and should be centered or transformed before clustering or computing fourier scores. The preferred method is SVD centering preceded by KNNImpute missing-value estimation, although mean-centering is a viable alternative. Hela Heat 42 degrees time course has 2 zeroes (the second is labeled "Cold 25 degrees 0h"). Normalization is by centering or zero transformation (average 2 zeroes) WI38 Crowding (1-3 days) was by plating cells at ~50%? confluence and allowing to age. The zeroes are the same as for WI38 Heat Shock (there are 2) Other time courses should be self-explanatory. They have 1-4 zeroes each and can be treated either by centering or zero-transforming. WI38 Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set Computed
transcription profiling by array
Stanford Microarray Database <firstname.lastname@example.org>, John Murray, Michael Whitfield
Diverse and specific gene expression responses to stresses in cultured human cells. Murray JI, Whitfield ML, Trinklein ND, Myers RM, Brown PO, Botstein D.