E-GEOD-4299 - Resveratrol-Induced Gene Expression Profiles in Human Prostate Cancer Cells
Submitted on 24 February 2006, released on 24 February 2006, last updated on 18 October 2011
Cell Culture and Treatments The LNCaP cell line was obtained from American Type Culture Collection (Manassas, VA) and maintained in RPMI medium with 10% fetal bovine serum (CDT; Hyclone Laboratories, Logan, UT) and penicillin/streptomycin (Mediatech, Herndon, VA) in an environment of 95% air and 5% CO2 at 370C. Upon reaching 75% confluence, cells were treated with either DMSO control or purified resveratrol at several concentrations (LKT Laboratories, St. Paul, MN), dissolved in DMSO, and incubated for varying lengths of time. Final concentration of DMSO in media was 0.01%. Total RNA was prepared from cells using TRIzol (Invitrogen Life Technologies, Carlsbad, CA). An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Computed
unknown experiment type
Stanford Microarray Database <email@example.com>, Samuel DePrimo, Sunita Jones
Resveratrol-induced gene expression profiles in human prostate cancer cells. Jones SB, DePrimo SE, Whitfield ML, Brooks JD.