E-GEOD-42833 - Analysis of transcriptional response to mutated Potyvirus Potato virus A that induces necrotic symptoms.

Status
Released on 23 March 2013, last updated on 31 March 2013
Organism
Nicotiana tabacum
Samples (8)
Array (1)
Protocols (8)
Description
Mutations introduced to a highly variable region in HCpro reduced HCpro-HIP2 interactions and virulence of PVA, and induced novel types of necrotic symptoms in the systemically infected leaves of Nicotiana species. Microarray-based analysis of gene expression revealed induction of large numbers of defence-related genes including jasmonic acid and ethylene inducible pathways in the leaves systemically infected by the mutated PVA. Three treaments: infection with wild type PVA (WT), infection with mutated PVA (MUT), and mock-inoculation (Mock). Six plants were treated for each treatment. Experiment was repeated three times. WT and MUT samples from all three experiments were hybridised on microarray. Mock samples from experiments 1 and 2 were hybridised as healthy controls.
Experiment type
transcription profiling by array 
Contacts
Jari P Valkonen, Minna-Liisa Rajamaki, Tuuli Haikonen, Yan-Ping Tian
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-42833.idf.txt
Sample and data relationshipE-GEOD-42833.sdrf.txt
Raw data (1)E-GEOD-42833.raw.1.zip
Processed data (1)E-GEOD-42833.processed.1.zip
Array designA-GEOD-10098.adf.txt
Links