E-GEOD-42789 - Gene expression in brain and liver produced by three different regimens of alcohol consumption in mice: Comparison with immune activation

Status
Released on 1 April 2013, last updated on 2 June 2014
Organism
Mus musculus
Samples (159)
Array (1)
Protocols (6)
Description
We investigated the molecular mechanisms of chronic alcohol consumption or lipopolysaccharide insult by gene expression profiling in prefrontal cortex and liver of C57BL/6J mice. We identified similar patterns of transcriptional changes in brain and liver among three different alcohol consumption tests and lipopolysaccharide injection. We also demonstrated distinct genomic consequences of different types of alcohol consumption. The microarray experiment was performed to compare gene expression changes induced by three separate paradigms of alcohol consumption and immune activation by lipopolysaccharide injection. The three tests of alcohol consumption were the continuous chronic two bottle choice (Chronic), two bottle choice available every other day (Chronic Intermittent) and limited access to one bottle of ethanol (Drinking in the Dark). All alcohol studies utilized 20% ethanol and each treatment group had it's own control group which received only water. The immune activation test consisted of 2 lipopolysaccharide injections (1 mg/kg i.p.) spaced one week apart, with animals being sacrificed one week after the last injection. Control animals received saline injections. All studies used female, adult mice.
Experiment type
transcription profiling by array 
Contacts
Elizabeth Osterndorff-Kahanek, Igor Ponomarev, R A Harris, Yuri A Blednov
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-42789.idf.txt
Sample and data relationshipE-GEOD-42789.sdrf.txt
Processed data (1)E-GEOD-42789.processed.1.zip
Array designA-MEXP-1174.adf.txt
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