E-GEOD-42222 - HeLa cells consist of two cell types, as evidenced by cytochemical staining for alkaline phosphatase activity: A possible model for cancer stem cell study

Status
Released on 23 April 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (2)
Array (1)
Protocols (7)
Description
Alkaline phosphatase (ALP) is known to be a marker for several somatic stem cells and cancer cells. We found that human squamous cell carcinoma HeLa cells are comprised by ALP-positive and negative cells. Single cell-derived colony assay revealed that the former cells are labile with respect to ALP activity, but the latter are stable. We cloned ALP-negative cells from the HeLa cells, and named H-1 clone. DNA microarray analysis revealed that gene expression pattern of H-1 cells is almost the same with that of their parental HeLa cells, but several genes for glycoprotein hormone alpha chain, ras-related and estrogen-regulated growth inhibitor, ALP, and Frizzled-10 was respectively 18.2, 9.6, 9.2 and 10.5–fold are upregulated in the HeLa cells. Although there is no evidence that the ALP-positive cells are cancer stem cells (CSCs) at present, HeLa cells comprised by ALP-positive and -negative cells may be a good model for CSC study in future. HeLa cells consist of two cell types, namely alkaline phosphatase (ALP)-positive and negative cells. To distinguish gene expression pattern between these two types of cells, microarray analysis was performed using HeLa cells (parental cell line; a mixture of ALP-positive and negative cells) and H-1 cells, ALP-negative cells derived from HeLa cells
Experiment type
transcription profiling by array 
Contacts
Emi Inada, Issei Saitoh, Masahiro Sato, Masato Ohtsuka, Naoko Kubota, Satoshi Watanabe, Shingo Nakamura, Takayuki Sakurai
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-42222.idf.txt
Sample and data relationshipE-GEOD-42222.sdrf.txt
Raw data (1)E-GEOD-42222.raw.1.zip
Processed data (1)E-GEOD-42222.processed.1.zip
Array designA-AFFY-44.adf.txt
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