E-GEOD-42102 - Proteomic and transcriptomic responses to thiamethoxam in the Sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)
Released on 15 July 2013, last updated on 5 September 2013
We investigated the transcriptional response to thiamethoxam in the Bemisia tabaci using Illumina sequencing technology. A total of 1,338 genes were differently expressed in the thiamethoxam-resistant whiteflies. All the B biotype whiteflies were maintained on cabbage. The susceptible strain (TH-S) was cultured without exposure to any chemical insecticides. Before the sample collected, almost 10,000 thiamethoxam-resistant adult whiteflies were treated with 2,000 mg/L thiamethoxam (~LC80) to eliminate the heterozygous individuals. Then the survivors were collected after 48 hours and designated as the TH-2000 sample. Then approximately 1,500 adult TH-S and TH-2000 were collected, respectively, The RNA was extracted and sequenced using Illumina HiSeq 2000.
RNA-seq of coding RNA
Nina Yang <email@example.com>, Wen Xie, Xin Yang, Xuguo Zhou, Youjun Zhang
Transcriptomic and proteomic responses of sweetpotato whitefly, Bemisia tabaci, to thiamethoxam. Yang N, Xie W, Yang X, Wang S, Wu Q, Li R, Pan H, Liu B, Shi X, Fang Y, Xu B, Zhou X, Zhang Y. , Europe PMC 23671574