ID_REF = VALUE = Genespring signal intensity
Quiagen Rneasy kit for isolated cells was used as per manufacturer's protocol
GeneChips were scanned using the Affymetrix GeneChip scanner 3000.
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug of total RNA
Data was analyzed with Genespring 9.0 software using default analysis settings. No minumium fluorescence cut-off was used for data analysis.
Following fragmentation, 15ug of cRNA were hybridized for 16 hrs at 45C on U133 2.0 Human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Whole PBMCs were isolated from blood and then CD11c+ cells were sorted using magnetic beads bound to human anti-CD11c+ antibody.