7 protocols
AccessionNameType
P-GSE42058-1
bioassay_data_transformation
ID_REF = VALUE = Genespring signal intensity
P-GSE42058-3
nucleic_acid_extraction
Quiagen Rneasy kit for isolated cells was used as per manufacturer's protocol
P-GSE42058-6
image_aquisition
GeneChips were scanned using the Affymetrix GeneChip scanner 3000.
P-GSE42058-4
labeling
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug of total RNA
P-GSE42058-7
feature_extraction
Data was analyzed with Genespring 9.0 software using default analysis settings. No minumium fluorescence cut-off was used for data analysis.
P-GSE42058-5
hybridization
Following fragmentation, 15ug of cRNA were hybridized for 16 hrs at 45C on U133 2.0 Human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
P-GSE42058-2
specified_biomaterial_action
Whole PBMCs were isolated from blood and then CD11c+ cells were sorted using magnetic beads bound to human anti-CD11c+ antibody.