E-GEOD-41913 - Effects of exercise on miRNA expression level in human NK Cells

Status
Released on 2 April 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (22)
Array (1)
Protocols (7)
Description
Exercise leads to a rapid change in the profile of gene expression in NK cells. We hypothesized that microRNA expression in circulating NK cells would also be affected by brief exercise. Eleven healthy men (20-29 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min rest at a constant work equivalent to about 77% of VO2max. A baseline blood sample was taken before the and immediately after the exercise. NK cells were isolated from PBMC using a negative magnetic cell separation methods (Miltenyi Biotec Kit #130-092-657 and the autoMACS® Pro Separator). Total RNA was extracted using TRIzol®. For this study we used Agilent Human miRNA microarrays V2 (total of 22 chips).
Experiment type
transcription profiling by array 
Contacts
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-41913.idf.txt
Sample and data relationshipE-GEOD-41913.sdrf.txt
Raw data (1)E-GEOD-41913.raw.1.zip
Processed data (1)E-GEOD-41913.processed.1.zip
Array designA-GEOD-10850.adf.txt
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