E-GEOD-41737 - MicroRNA-27a regulates lipid metabolism and inhibits hepatitis C virus replication in human hepatoma cells

Status
Released on 5 June 2013, last updated on 13 May 2014
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (5)
Description
Gene expression profiling was carried out in Huh-7.5 cells in which miR-27a was over- or under-expressed. Transfection of cells with pre-miR-27a and pre-miR-control, or anti-miR-27a and anti-miR-control enabled down- and up-regulated genes to be determined, respectively. Replication and infectivity of the lipotrophic hepatitis C virus (HCV) is regulated by cellular lipid status. Among differentially expressed micro (mi)RNAs, we found that miR-27a was preferentially expressed in HCV-infected compared with hepatitis B virus (HBV)-infected liver. Gene expression profiling of Huh-7.5 cells showed that miR-27a regulates lipid metabolism by targeting the lipid synthetic transcriptional factor, RXRα, and the lipid transporter, ABCA1 Carrying out a Target Scan (Release 5.2) of miR-27a predicted 921 candidate target genes, and functional gene ontology enrichment analysis of these genes by MetaCore (Thomson Reuters, NY) showed that miR-27a could target the signaling pathways of cytoskeleton remodeling and lipid metabolism . To examine whether these signaling pathways were regulated by miR-27a, gene expression profiling was carried out in Huh-7.5 cells in which miR-27a was over- or under-expressed. Transfection of cells with pre-miR-27a and pre-miR-control, or anti-miR-27a and anti-miR-control enabled down- and up-regulated genes to be determined, respectively. Huh-7.5 cells with miR-27a over- or under-expressed
Experiment type
transcription profiling by array 
Contacts
Masao Honda <mhonda@m-kanazawa.jp>, Akito Sakai, Hikari Okada, MinKyung Yi, Rika Horii, Risa Watanabe, Seishi Murakami, Shuichi Kaneko, Stanley M Lemon, Takayoshi Shirasaki, Taro Yamashita, Tetsuro Shimakam, Yoshio Sakai
Citation
MicroRNA-27a regulates lipid metabolism and inhibits hepatitis C virus replication in human hepatoma cells. Shirasaki T, Honda M, Shimakami T, Horii R, Yamashita T, Sakai Y, Sakai A, Okada H, Watanabe R, Murakami S, Yi M, Lemon SM, Kaneko S. , Europe PMC 23449803
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-41737.idf.txt
Sample and data relationshipE-GEOD-41737.sdrf.txt
Raw data (1)E-GEOD-41737.raw.1.zip
Processed data (1)E-GEOD-41737.processed.1.zip
Array designA-AFFY-44.adf.txt
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