8 protocols
AccessionNameType
P-GSE41520-7
image_aquisition
Standard Illumina scanning protocol
P-GSE41520-8
feature_extraction
The data were first raw-filtered in order to identify and remove probesets that were not detected and then normalised using robust spline normalization with FlexArray v1.6
P-GSE41520-1
bioassay_data_transformation
ID_REF = VALUE = log2 robust spline normalized
P-GSE41520-6
hybridization
Standard Illumina hybridization protocol
P-GSE41520-5
labeling
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
P-GSE41520-2
specified_biomaterial_action
Mice were infected for 6h with 5 x 10^7 CFU of Streptococcus suis strain 89-1591 or mock-infected with bacterial growth medium (THB).
P-GSE41520-4
nucleic_acid_extraction
RNA was homogenized from RNAlater-stabilized spleen tissus, extracted and DNAse I treated using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
P-GSE41520-3
grow
Bacterial culture was prepared by transferring 10 μl of 1/1,000 dilutions of 8-h cultures into 30 ml of THB, which was incubated for 16 h at 37°C without agitation. Stationary-phase bacteria were washed twice in PBS (pH 7.3). The bacterial pellet was then resuspended and adjusted to a concentration of 5 × 10^8 c.f.u. ml-1.