E-GEOD-41497 - Development of Helicobacter pylori microarrays to test pathogenicity of clinical isolates
Released on 5 August 2013, last updated on 12 August 2013
To distinguish between Helicobacter pylori isolates that may cause greater disease in patients, we used whole genome expression profiling as a platform to study host-pathogen interactions and identify gene signatures associated with isolates from patients with higher cancer risk. Expression profiles were studied for 3 clinical isolates from a region of high gastric cancer incidence (PZ5056, PZ5080, PZ5086) in Colombia and 3 isolates from a region with low gastric cancer incidence in Colombia (PZ5004, PZ5024, PZ5026). Each experiment was done in triplicate by infecting monolayers of gastric epithelial cells for 1 hour with the isolates.
transcription profiling by array
Alexander Sheh, D S Merrell, James G Fox, Keith T Wilson, Pelayo Correa, Rupesh Chaturvedi
Phylogeographic origin of Helicobacter pylori determines host-adaptive responses upon coculture with gastric epithelial cells. Sheh A, Chaturvedi R, Merrell DS, Correa P, Wilson KT, Fox JG. , Europe PMC 23630959