5 protocols
AccessionNameType
P-GSE41438-1
normalization data transformation protocol
Bioconductor software, log2 RMA normalization. ID_REF = VALUE = Log2 RMA-normalized signal
P-GSE41438-5
array scanning protocol
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 through the University of Washington Functional Genomics Laboratory in the Center for Ecogenetics and Environmental Health (http://depts.washington.edu/ceeh/).
P-GSE41438-4
hybridization protocol
The standard hybridization protocol was used as recommended by Affymetrix.
P-GSE41438-3
labelling protocol
For microarray hybridization, RNA was processed for hybridization to Affymetrix Mouse Gene 1.0 ST Arrays through a core facility in the Functional Genomics Laboratory in the Center for Ecogenetics and Environmental Health at the University of Washington according to the manufacturer's instructions.
P-GSE41438-2
nucleic acid extraction protocol
RNA extraction was performed by the CEEH Genomics Core Laboratory followed by the manufacturer’s protocols using the GeneChip platform by Affymetrix.