E-GEOD-41164 - Expression data from splenic B-cells isolated from DmU50(HG-b) mice or wild-type C57BL/6J

Status
Released on 26 March 2013, last updated on 2 June 2014
Organism
Mus musculus
Samples (2)
Array (1)
Protocols (8)
Description
Box C/D-type small nucleolar RNAs (snoRNAs) are functional RNAs responsible for mediating 2’-O-ribose methylation of ribosomal RNAs (rRNAs) within the nucleolus. Previously, in relation to a novel chromosomal translocation in a human B-cell lymphoma, we identified U50HG, a non-protein-coding gene that hosted a box C/D-type U50 snoRNA within its intron. To investigate the physiological importance of the U50 snoRNA and its involvement in tumorigenesis, we generated a mouse model deficient in mouse U50 (mU50) snoRNA expression without altering the expression of mouse mU50 host-gene, mU50HG-b. The established mU50 snoRNA-deficient mice showed a significant reduction of mU50 snoRNA expression and the corresponding target rRNA methylation in various organs. Lifelong phenotypic monitoring showed that the mU50-deficient mice looked almost normal without accelerated tumorigenicity; however, a notable difference was the propensity for anomalies in the lymphoid organs. B-cells were isolated from spleens of DmU50(HG-b) mice or wild-type C57BL/6J with antibody-conjugated magnetic beads system (Myltenyi Biotec). Total RNA was purified with QIAGEN RNeasy Micro kit. Affymetrix GeneChip® Microarrays (Mouse Expression 430 2.0 Array) were used.
Experiment type
transcription profiling by array 
Contacts
Yuuichi Soeno <patho-aoba@tky.ndu.ac.jp>, Kazuya FUJITA, Takaaki AOBA, Yuji TAYA, Yuuichi SOENO
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-41164.idf.txt
Sample and data relationshipE-GEOD-41164.sdrf.txt
Raw data (1)E-GEOD-41164.raw.1.zip
Processed data (1)E-GEOD-41164.processed.1.zip
Array designA-AFFY-45.adf.txt
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