E-GEOD-40978 - Long non-coding RNAs act as novel mediators of Innate Immunity

Status
Released on 26 August 2013, last updated on 19 September 2013
Organism
Mus musculus
Samples (4)
Protocols (4)
Description
Recent advances in genome technologies have uncovered thousands of long non-coding RNAs (lncRNAs) whose function in the immune system has been largely unexplored. Members of the Toll-like receptor (TLR) family activate an inducible program of inflammatory genes important in host defenses. Here we provide evidence that TLRs induce the expression of many lncRNAs. One of these (lncRNA-Cox2) modulates the expression of hundreds of TLR2-inducible genes. Functional studies identified this lncRNA as capable of both activation and repression of distinct groups of TLR-induced genes. Transcriptional repression mediated by lncRNA-Cox2 requires heterogeneous ribonucleoprotein A/B and A2/B1, binding partners for lncRNA-Cox2. Collectively, these studies identify lncRNA-Cox2 as a broad-acting component of the regulatory circuit that controls the TLR-induced inflammatory response. Examination of Mus musculus (C57BL/6 background) gene exression changes following stimulation with Pam3Cys4 in presence or absence of shRNA specifically targetting lncRNA-COX2
Experiment type
RNA-seq of coding RNA 
Contacts
Daniel Aiello <Daniel.Aiello@umassmed.edu>, Katherine A Fitzgerald, Susan Carpenter
Citation
A long noncoding RNA mediates both activation and repression of immune response genes. Carpenter S, Aiello D, Atianand MK, Ricci EP, Gandhi P, Hall LL, Byron M, Monks B, Henry-Bezy M, Lawrence JB, O'Neill LA, Moore MJ, Caffrey DR, Fitzgerald KA. , Europe PMC 23907535
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-40978.idf.txt
Sample and data relationshipE-GEOD-40978.sdrf.txt
Processed data (1)E-GEOD-40978.processed.1.zip
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