E-GEOD-4097 - Transcription profiling of human SHEP-SF cells expressing AICD and Fe65
Submitted on 25 January 2006, released on 22 June 2007, last updated on 27 March 2012
The amyloid precursor protein (APP) plays a central role in the pathogenesis of Alzheimer’s disease (AD). Processing of APP by β- and γ-secretase activities results in the production of ß-amyloid (Aß), the main constituent of Alzheimer plaques, but also in the generation of the APP intracellular domain (AICD). Recently, it has been demonstrated that AICD has transactivation potential, however, the targets of AICD dependent gene regulation and hence the physiological role of AICD remain largely unknown. In this work we analysed transcriptome changes during AICD dependent gene regulation using a human neural cell culture system inducible for expression of AICD, its co-activator Fe65, or the combination of both. Induction of AICD was associated with increased expression of genes with known function in the organization and dynamics of the actin cytoskeleton as well as genes involved in the regulation of apoptosis.
transcription profiling by array, co-expression, genetic modification
Modulation of gene expression and cytoskeletal dynamics by the amyloid precursor protein intracellular domain (AICD). Müller T, Concannon CG, Ward MW, Walsh CM, Tirniceriu AL, Tribl F, Kögel D, Prehn JH, Egensperger R. Mol Biol Cell 18(1):201-10, Europe PMC 17093061