E-GEOD-40839 - Expression data from fibroblasts cultured from normal and fibrotic human lung tissue

Status
Released on 5 August 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (21)
Array (1)
Protocols (7)
Description
Pulmonary fibrosis develops as a consequence of environmentally induced lung injury and/or an inherent disease susceptibility causing fibroblast activation, proliferation and extracellular matrix deposition. The study was undertaken to characterise global gene expression in pulmonary fibroblasts to better understand the mechanisms underlying the fibrotic fibroblast phenotype. Gene expression was evaluated in lung fibroblasts derived from ten controls (normal periphery of resected tumor), open lung biopsies from eight patients with interstitial lung disease associated with systemic sclerosis (fibrotic non specific interstitial pneumonia pattern on biopsy), and from three patients with usual interstitial pneumonia. Lung fibroblasts were grown to confluence in DMEM with 10% fetal calf serum. At confluence, lung fibroblasts were serum-deprived for 44 hours in the presence of fibroblast growth medium with the addition of 0.1% bovine serum albumin (Sigma).
Experiment type
transcription profiling by array 
Contacts
Carmel Stock <c.stock@imperial.ac.uk>, Andrew G Nicholson, Athol U Wells, Carmel J Stock, Christopher P Denton, David J Abraham, Elisabetta A Renzoni, George Bou-Gharios, Gisela E Lindahl, Michael E Dusmet, Xu Shi-Wen
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-40839.idf.txt
Sample and data relationshipE-GEOD-40839.sdrf.txt
Raw data (1)E-GEOD-40839.raw.1.zip
Processed data (1)E-GEOD-40839.processed.1.zip
Array designA-AFFY-33.adf.txt
Links