E-GEOD-40695 - Expression data from NSCs treated with REST inhibitor X5050

Status
Released on 1 October 2013, last updated on 7 October 2013
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
Description
REST has been initially described as a repressor of neuronal genes in non-neuronal cells by binding to its recognition sequence RE1. Over-activation of this factor has been shown in several diseases such as Huntington disease or central nervous system cancers. High-throughput screening of a library of 6,984 compounds with luciferase-assay measuring REST activity in neural derivatives of human embryonic stem cells led to the identification of one benzoimidazole-5-carboxamide derivative (X5050) that inhibited REST silencing in a RE1-dependent manner. Differential transcriptomic analysis revealed the upregulation of neuronal genes targeted by REST. RNA was extracted from 6 samples, corresponding to 3 independent cultures of NSC SA-01, each one treated either with DMSO (0.1 % final) or with X5050 (100 µM final). RNA was isolated using RNeasy Mini kit with DNase I digestion and genome-wide gene expression profiling was performed by hybridization on Affymetrix microarrays
Experiment type
transcription profiling by array 
Contacts
Florent Dumont <florent.dumont@inserm.fr>, Anselme L Perrier, Jérémie Charbord
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-40695.idf.txt
Sample and data relationshipE-GEOD-40695.sdrf.txt
Raw data (1)E-GEOD-40695.raw.1.zip
Processed data (1)E-GEOD-40695.processed.1.zip
Array designA-AFFY-141.adf.txt
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