E-GEOD-40512 - Transcription profiling by array of KOPT-K1 T-ALL cells after treatment with PD 0332991

Status
Released on 16 October 2012, last updated on 30 April 2015
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (8)
Description
D-cyclins represent components of cell cycle machinery. To test the efficacy of targeting D-cyclins in cancer treatment, we engineered mouse strains which allow acute and global ablation of individual D-cyclins in a living animal. Ubiquitous shutdown of cyclin D1 or inhibition of cyclin D associated kinase activity in mice bearing ErbB2-driven mammary carcinomas halted cancer progression and triggered tumor-specific senescence, without compromising the animals' health. Ablation of cyclin D3 in mice bearing T-cell acute lymphoblastic leukemias (T-ALL) triggered tumorspecific apoptosis. Such selective killing of leukemic cells can be also achieved by inhibiting cyclin D associated kinase activity in mouse and human T-ALL models. Hence, contrary to what one might expect from ablation of a cell cycle protein, acute shutdown of a D-cyclin leads not only to cell cycle arrest, but it also triggers tumor cell senescence or apoptosis, and it affects different tumor types through distinct cellular mechanisms. Inhibiting cyclin D-activity represents a highly-selective anticancer strategy which specifically targets cancer cells without significantly affecting normal tissues. A human T-ALL cell line KOPTK1 cells were cultured in the presence of the CDK4/6 inhibitor PD 0332991 (PD; 1 microM) or vehicle (VO) for 48 hrs. Experiment was done in biological triplicate. A total of 6 RNA samples (3 vehicle treated and 3 PD 0332991 treated samples) were used for microarray expression analysis.
Experiment types
transcription profiling by array, compound treatment design
Contacts
Takaomi Sanda <takaomi_sanda@dfci.harvard.edu>, Amanda L Christie, Andrew L Kung, Harald von Boehmer, Joanna Stefano, Per Hydbring, Piotr Sicinski, Sabina Signoretti, Thomas Look, Xiaoyu Li, Yoon J Choi
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-40512.idf.txt
Sample and data relationshipE-GEOD-40512.sdrf.txt
Raw data (1)E-GEOD-40512.raw.1.zip
Processed data (1)E-GEOD-40512.processed.1.zip
Array designA-AFFY-44.adf.txt
Links