E-GEOD-40384 - siRNA Knockdown of SWI/SNF complex proteins in prostate cancer cell lines

Status
Released on 20 July 2013, last updated on 2 June 2014
Organism
Homo sapiens
Samples (24)
Array (1)
Protocols (7)
Description
Here we performed transcriptional profiling of the prostate cancer cell lines LNCaP and 22Rv1 comparing non-targeting siRNA treatment versus siRNAs targeting SWI/SNF complex proteins (SMARCA2, SMARCA4, and SMARCB1). Goal was to determine the effect of SWI/SNF knockdown on gene expression in prostate cancer. Two-condition experiment: non-targeting siRNA versus SWI/SNF-siRNA treated cells. Three SWI/SNF proteins were targeted: SMARCA2, SMARCA4, and SMARB1. Biological replicates: 1 control replicate, 2 treatment replicates per SWI/SNF protein. Technical replicates: 1 replicate per SWI/SNF protein. Cell lines: 22Rv1 and LNCaP.
Experiment type
transcription profiling by array 
Contacts
Matthew Iyer <mkiyer@med.umich.edu>, John R Prensner, Matthew K Iyer, Xiaojun Jing
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-40384.idf.txt
Sample and data relationshipE-GEOD-40384.sdrf.txt
Raw data (1)E-GEOD-40384.raw.1.zip
Processed data (1)E-GEOD-40384.processed.1.zip
Array designA-AGIL-28.adf.txt
Links